rabbit anti pdgfrβ pab  (Bioss)

Journal: Veterinary Sciences

Article Title: PDGFRβ Expression Across Canine AGASAC Subtypes and Metastases: Morphologic Insights and Possible Therapeutic Implications †

doi: 10.3390/vetsci12121122

Figure Lengend Snippet: Microscopic images of canine AGASAC growth patterns. ( A , B ) Pure solid pattern: ( A ) Lobules of neoplastic cells with scant stroma, surrounded by desmoplasia; ( B ) PDGFRβ is variably expressed in neoplastic cells’ cytoplasm and membrane. Blood vessel endothelial cells and stromal CAFs are PDGFRβ-positive. ( C , D ) Pure tubular pattern: ( C ) Neoplastic cells in well-formed tubules; ( D ) PDGFRβ is strongly expressed in the tubular epithelium’s cytoplasm and endothelial cells. ( E , F ) Mixed pattern: ( E ) Areas with tubular and solid growth; ( F ) PDGFRβ expression is heterogeneous, with strong labeling in tubules and weaker in solid areas. CAFs are visible in the stroma. ( G , H ) Comedo-carcinoma pattern: ( G ) Solid sheets with necrosis; ( H ) neoplastic cells and CAFs have moderate cytoplasmic PDGFRβ. ( I , J ) Neuroendocrine-like pattern (rosettes and packets): ( I ) Small, scattered to confluent packets; ( J ) PDGFRβ varies, often labeling scattered cells from strong to weak. ( A , C , E , G , I )—H&E stain; ( B , D , F , H , J )—PDGFRβ expression, evaluated by immunohistochemistry with DAB chromogen.

Article Snippet: PDGFRβ expression was evaluated on 4 μm paraffin sections of the primary AGASAC and metastases, mounted on poly-L-lysine-coated slides [ , ], and stained with rabbit polyclonal anti-human PDGFRβ primary antibody (3162S, Cell Signaling Technology, Danvers, MA, USA).

Techniques: Membrane, Expressing, Labeling, Staining, Immunohistochemistry

Journal: Veterinary Sciences

Article Title: PDGFRβ Expression Across Canine AGASAC Subtypes and Metastases: Morphologic Insights and Possible Therapeutic Implications †

doi: 10.3390/vetsci12121122

Figure Lengend Snippet: PDGFRβ immunohistochemical expression in primary AGASACs and corresponding metastasis. ( A ) Primary AGASAC with tubular growth pattern demonstrating diffuse, intense, cytoplasmic PDGFRβ expression. ( B ) Corresponding metastatic lesion in the sacral lymph node, characterized by a shift toward a more solid growth pattern and reduced, faint PDGFRβ staining.

Article Snippet: PDGFRβ expression was evaluated on 4 μm paraffin sections of the primary AGASAC and metastases, mounted on poly-L-lysine-coated slides [ , ], and stained with rabbit polyclonal anti-human PDGFRβ primary antibody (3162S, Cell Signaling Technology, Danvers, MA, USA).

Techniques: Immunohistochemical staining, Expressing, Staining